general estradiol e2 elisa kit  (Bioss)

Journal: EMBO Reports

Article Title: An insulin receptor activity surge in follicle cells drives vitellogenesis by upregulating CrebA

doi: 10.1038/s44319-025-00672-6

Figure Lengend Snippet: ( A ) Alignment of the bZIP domains of CrebA, CREB3, and CREB3L1-4. Within this domain, CREB3 and CREB3L1-4 share 53.1, 60.9, 70.3, 54.7, and 51.6% identities with CrebA, respectively. ( B ) Phylogenetic tree based on the alignment of the bZIP domains. ( C ) RT-qPCR shows the increases of CREB3 , CREB3L1 and CREB3L2 mRNA in KGN cells treated with FOXO1 siRNAs. N = 3 biological replicates for each experimental group. Error bars represent mean ± SEM. One-way ANOVA p values = 0.005 (denoted as **), 0.01 (**), 0.02 (*), 0.047 (*), 0.93 (ns), 0.03 (*), 0.008 (**), and 0.0002 (***) for the significance marks from left to right respectively. ( D ) GO/KEGG terms enriched with genes that were downregulated in KGN cells treated with a CREB3L2 siRNA. ( E – G ) RT-qPCR, Western blot and ELISA analyses show that both CYP19A1 protein and 17-beta-estradiol (E2) were reduced by CREB3L2 siRNA treatments. N = 3 biological replicates for each experiment. Error bars represent mean ± SEM. One-way ANOVA p = 0.008 (**) and 0.009 (**) for qPCR, and p = 0.04 (*) and 0.04 (*) for ELISA, respectively. ( H – J ) RT-qPCR, Western blot and ELISA analyses show that knockdown of FOXO1 counteracted the effects of CREB3L2 siRNAs on CREB3L2 mRNA, CYP19A1 protein and E2 levels. N = 3 biological replicates for each experiment. Error bars represent mean ± SEM. One-way ANOVA p = 0.01 (*), 0.01 (*), 0.12 (ns), and 0.34 (ns) for qPCR, and p = 0.06 (ns) and 0.84 (ns) for ELISA, respectively. .

Article Snippet: E2 ELISA Kit , ABclonal , RK00651.

Techniques: Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Knockdown

Journal: Current Research in Physiology

Article Title: Intergenerational protein deficiency and adolescent reproductive function of subsequent female generations (F 1 and F 2 ) in rat model

doi: 10.1016/j.crphys.2021.12.003

Figure Lengend Snippet: Effect of perinatal protein deficiency on A: reproductive response in the different diet groups, at F 1 and F 2 generations. A: Fertility index, B: Mean Serum Estradiol and Progesterone levels on gestation days 6 and 19, Data are expressed as mean ± SEM, n = 6 in each group. Control (standard rat chow containing 16–18% Protein), 21%PD (Upgraded daily recommended intake), 5%PD (Protein deficient diet), 10%PD (Mild protein-deficientdiet). E2-Estradiol, PROG- Progesterone, GD6 &GD19; Gestation Day 6 and 19. a; significantly different from 21% diet group, b; significantly different from the control diet group, c; significantly different from the 10% diet group (*P < 0.05, **P < 0.001, ***P < 0.0001).

Article Snippet: F 1 -generation serum estradiol and progesterone, Follicle Stimulating Hormone, Luteinizing Hormone, Testosterone and cortisol levels were measured in each diet group at puberty (specifically the Oestrus phase) by EIA kit (Monobind Inc. Lake forest USA), using hormone standards and the specific antibodies following manufacturer's instructions of kit.

Techniques:

Journal: Current Research in Physiology

Article Title: Intergenerational protein deficiency and adolescent reproductive function of subsequent female generations (F 1 and F 2 ) in rat model

doi: 10.1016/j.crphys.2021.12.003

Figure Lengend Snippet: Effect of protein deficiency on the onset of puberty in each of the diet groups, across F 1 and F 2 generations. A: The Plot of percentage vaginal opening at different postnatal days (between PND 32–80), at F1. A: The Plot of percentage vaginal opening at different postnatal days (between PND 32–80), at F 1 . B: The Plot of percentage vaginal opening at different postnatal days (between PND 32–80), at F 2 . The onset of puberty taken as the postnatal date of complete removal of membrane sheath of the vaginal orifice. Data are expressed as mean ± SEM, Control (standard rat chow containing 16–18% Protein), 21%PD (upgraded daily recommended intake), 5%PD (Protein deficient diet), 10%PD (Mild protein-deficient diet). A statistically significant difference between individual group and 5% PD (*P < 0.05) and the hypothetical average age of vagina opening (PND 36). PD: Protein Diet. PND: Postnatal Days .

Article Snippet: F 1 -generation serum estradiol and progesterone, Follicle Stimulating Hormone, Luteinizing Hormone, Testosterone and cortisol levels were measured in each diet group at puberty (specifically the Oestrus phase) by EIA kit (Monobind Inc. Lake forest USA), using hormone standards and the specific antibodies following manufacturer's instructions of kit.

Techniques:

Journal: Current Research in Physiology

Article Title: Intergenerational protein deficiency and adolescent reproductive function of subsequent female generations (F 1 and F 2 ) in rat model

doi: 10.1016/j.crphys.2021.12.003

Figure Lengend Snippet: Effect of perinatal dietary protein deficiency on oestrus cyclicity across F 1 and F 2 -generations cycle phase in each of the diet groups. A: Percentage time spent in each cycle phase, B: Percentage persistence in diestrus phase, C: Average length of each cycle phase. Data are expressed as mean ± SEM, n = 6, Control (standard rat chow containing 16–18% Protein), 21%PD (upgraded daily recommended intake), 5% (Protein deficient diet), 10% (Mild protein-deficient diet). A statistically significant difference between individual group and 5% PD (*P < 0.05) and the hypothetical value of 7 day cycle length. A Persistent cycle length > 7days reflects an irregular cycle. PD: Protein Deficient Diet. a < significantly different from 21% diet group > b < significantly different from the control c < significantly different from the 10% diet group> (*P < 0.05, **P < 0.001, ***P < 0.0001).

Article Snippet: F 1 -generation serum estradiol and progesterone, Follicle Stimulating Hormone, Luteinizing Hormone, Testosterone and cortisol levels were measured in each diet group at puberty (specifically the Oestrus phase) by EIA kit (Monobind Inc. Lake forest USA), using hormone standards and the specific antibodies following manufacturer's instructions of kit.

Techniques:

Journal: Current Research in Physiology

Article Title: Intergenerational protein deficiency and adolescent reproductive function of subsequent female generations (F 1 and F 2 ) in rat model

doi: 10.1016/j.crphys.2021.12.003

Figure Lengend Snippet: Effect of protein deficiency on serum puberty sex hormone levels during oestrus phase of the F 1 and F 2 generation in different diet groups. A: Luteinizing Hormone (LH), and Follicle-stimulating Hormone (FSH), B: Progesterone and Estradiol, C: Cortisol and Testosterone. Data are expressed as mean ± SEM, Control (standard rat chow containing 16–18% Protein), 21%PD (upgraded daily recommended intake), 5% (Protein deficient diet), 10% (Mild protein-deficient diet). a < significantly different from 21% diet group and or control > b < significantly different from the 10% diet group> (*P < 0.05, **P < 0.001, ***P < 0.0001). LH: Luteinizing hormone, FSH; Follicle Stimulating Hormone, PG; Progesterone, E2; Estradiol.

Article Snippet: F 1 -generation serum estradiol and progesterone, Follicle Stimulating Hormone, Luteinizing Hormone, Testosterone and cortisol levels were measured in each diet group at puberty (specifically the Oestrus phase) by EIA kit (Monobind Inc. Lake forest USA), using hormone standards and the specific antibodies following manufacturer's instructions of kit.

Techniques:

Journal: Current Research in Physiology

Article Title: Intergenerational protein deficiency and adolescent reproductive function of subsequent female generations (F 1 and F 2 ) in rat model

doi: 10.1016/j.crphys.2021.12.003

Figure Lengend Snippet: Effect of protein deficiency on the morphometric analysis of ovarian follicles in various diet groups at F 1 and F 2 generations A: Follicular Phase count at F 1 , B: Follicular Phase count at F 2 , C: Follicle diameter at F 1 and F 2 , D: Granulosa layer thickness at F 1 and F 2 , E: Theca layer thickness at F 1 and F 2 Data are expressed as mean ± SEM, n = 6, Control (standard rat chow containing 16–18% Protein), 21%PD (upgraded daily recommended intake), 5% (Protein deficient diet), 10% (Mild protein-deficient diet). a; significantly different from the 21% diet group, b; significantly different from the control diet group, c; significantly different from the 10% diet group, F 1 and F 2 are first and second generations respectively. (*P < 0.05, **P < 0.001, ***P < 0.0001).

Article Snippet: F 1 -generation serum estradiol and progesterone, Follicle Stimulating Hormone, Luteinizing Hormone, Testosterone and cortisol levels were measured in each diet group at puberty (specifically the Oestrus phase) by EIA kit (Monobind Inc. Lake forest USA), using hormone standards and the specific antibodies following manufacturer's instructions of kit.

Techniques: